Annexin III antibody

Name: Annexin III antibody
Brand: GeneTex
SKU: GTX103330
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-Annexin III antibody used in Western Blot (WB). GTX103330

Anti-Annexin III antibody used in IHC (Paraffin sections) (IHC-P). GTX103330

Cat. No. GTX103330

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Rat
Package	100 μl ($399), 25 μl ($169)

See all Annexin III products

View product citations for antibody GTX103330 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:20000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

A549 , HCT116

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Annexin III. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174712

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

annexin A3 , ANX3

Background

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation. [provided by RefSeq]

Database

Gene ID: 306 ANXA3
UniProt: P12429 ANXA3

Research Area

DATA IMAGES

GTX103330 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: HCT116
10% SDS PAGE
GTX103330 diluted at 1:10000

GTX103330 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using Annexin III(GTX103330) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Chai X et al. Transl Cancer Res 2022; 11 (6) : 1644-1651 Expression of HIF-1α, ANXA3, CD133 and their associations with clinicopathological parameters in human colon carcinoma.

Kunli Du et al. Transl Cancer Res 2020; 9 (12) : 7440-7449 ANXA3 is upregulated by hypoxia-inducible factor 1-alpha and promotes colon cancer growth.

Application : IHC-P

Reactivity : Human

Chang SJ et al. Arch Biochem Biophys 2018; 647 : 10-32 Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma.

Application : WB

Reactivity : Human

Huang HJ et al. Mol Biosyst 2014; (Epub) Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.

Application : WB

Reactivity : Human

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