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Histone H3 antibody

Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148
Anti-Histone H3 antibody used in Western Blot (WB). GTX122148
Anti-Histone H3 antibody used in ChIP assay (ChIP assay). GTX122148
Anti-Histone H3 antibody used in Immunoprecipitation (IP). GTX122148
Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148
Anti-Histone H3 antibody used in Western Blot (WB). GTX122148
Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148
Anti-Histone H3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122148
Anti-Histone H3 antibody used in Western Blot (WB). GTX122148
Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148

Cat. No. GTX122148

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, ChIP assay, IHC

Reactivity

Human, Mouse, Rat, Drosophila, Pig, Monkey, Fungi, Golden Syrian Hamster, Rice
Package
100 μl ($399),
25 μl ($169)
PRODUCT

Summary

Histone H3 antibody detects histone H3, a core component of the histone octamer in the context of the nucleosome. Like other histones, H3 is posttranslationally modified to impact epigenetic gene regulation. It is also highly conserved in eukaryotes, and therefore is used as an internal control for western blots or as a nuclear marker when conducting immunostaining.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
IHC Assay dependent
Not tested in other applications.

Positive Control

Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Human (100%)| Mouse (100%)| Rhesus Monkey (100%)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10633308

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148

GTX122148 IHC-P Image

Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Green: Histone H3 antibody (GTX122148) diluted at 1:500.

Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3 antibody used in Western Blot (WB). GTX122148

GTX122148 WB Image

Histone H3 antibody detects Histone H3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX122148) diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H3 antibody used in ChIP assay (ChIP assay). GTX122148

GTX122148 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-Histone H3 antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.

Anti-Histone H3 antibody used in Immunoprecipitation (IP). GTX122148

GTX122148 IP Image

Histone H3 antibody immunoprecipitates Histone H3 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of Histone H3 by 3 μg of Histone H3 antibody (GTX122148) 15% SDS-PAGE The immunoprecipitated Histone H3 protein was detected by Histone H3 antibody (GTX122148) diluted at 1:5000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148

GTX122148 IHC-P Image

Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Histone H3 antibody (GTX122148) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3 antibody used in Western Blot (WB). GTX122148

GTX122148 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX122148) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148

GTX122148 IHC-P Image

Histone H3 antibody detects Histone H3 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H3 antibody (GTX122148) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122148

GTX122148 ICC/IF Image

Histone H3 antibody detects Histone H3 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Histone H3 protein stained by Histone H3 antibody (GTX122148) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Histone H3 antibody used in Western Blot (WB). GTX122148

GTX122148 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX122148) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148

GTX122148 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Histone H3(GTX122148) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

Histone H3 antibody Cat. No. GTX122148

Rating ( Average 4.5 based on 11 users reviews)
Application
Western Blot(WB) ( Average 4.4 based on 8 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Dec-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : Jurkat cells
Amount used : 50 μg
Blocking : 5% non-fat milk in TBST, 1°C, 25Hr
Primary Antibody : 1:1000, 4°C, 18Hr
Date : Anonymous submitted on 17-May-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : human PBMC
Amount used : 10μg
Blocking : 5% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:3000, °C, Hr
Figure Note : WCL : wholc cell lysate
CE : cytoplasmic fraction
NE : nuclear fraction
Date : Anonymous submitted on 22-Sep-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : SW1353 cells
Amount used : 25μg
Blocking : Block Ace, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 12Hr
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Sample Species : Dm
Figure Note : The data was peer reviewed and published in the journal PLoS Genet in 2014. PMID: 25393278
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Virol in 2017. PMID: 28855246
Date : Anonymous submitted on 27-Dec-2020
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Mouse lung tissue
Blocking : 4% BSA in PBS, 25°C, 1Hr
Fixation : Formaldehide
Permeabilization /Antigen retrieval : Citrate buffer, pH6.0, 90°C, 0.6Hr
Primary Antibody : 1:500, 4°C, 16Hr
Figure Note : Histone H3 accumulation in nucleus is shown in brown. Rabbit IgG is used as negative control.
Date : Anonymous submitted on 05-Oct-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 10 μg
Blocking : 2.5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Date : Anonymous submitted on 05-Oct-2019
Score :
Application Tested : WB
Sample Species : Golden Syrian Hamster
Sample : BHK21 cells
Amount used : 5 μg
Blocking : 2.5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Date : Anonymous submitted on 23-May-2014
Score :
Application Tested : WB
Sample Species : Rice
Sample : rice leaf tissue
Amount used : 50μg
Blocking : 5% milk in tris buffer, 4°C, 1Hr
Primary Antibody : 1:2500, 4°C, 16Hr
Date : Anonymous submitted on 10-Mar-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : primary adipocyte cells
Amount used : 10μg
Blocking : 5% nonfat milk in TBST(1xTBS, 0.1%Tween 20), 25°C, 3Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Date : Anonymous submitted on 22-Dec-2012
Score :
Application Tested : WB
Sample Species : Mk
Sample : Vero E6 cells
Amount used : 100μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Application Reference
Simona Dedoni et al. Int J Mol Sci 2021; 22 (15)
Application : WB
Reactivity : Human
Chen CY et al. Int J Mol Sci 2020; 22 (1)
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169