ACAT1 antibody [N1N3]

Name: ACAT1 antibody [N1N3]
Brand: GeneTex
SKU: GTX102637
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-ACAT1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102637

Anti-ACAT1 antibody [N1N3] used in Western Blot (WB). GTX102637

Anti-ACAT1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX102637

Cat. No. GTX102637

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Zebrafish
Package	100 μl ($399), 25 μl ($169)

See all ACAT1 products

View product citations for antibody GTX102637 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , Molt-4

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ACAT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949561

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acetyl-CoA acetyltransferase 1 , ACAT , MAT , T2 , THIL

Cellular Localization

Mitochondrion

Background

This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq]

Database

Gene ID: 38 ACAT1
UniProt: P24752 ACAT1

Research Area

DATA IMAGES

GTX102637 ICC/IF Image

ACAT1 antibody [N1N3] detects ACAT1 protein at mitochondria by immunofluorescent analysis.
Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: ACAT1 protein stained by ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX102637 ICC/IF Image

ACAT1 antibody [N1N3] detects ACAT1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ACAT1 protein stained by ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

GTX102637 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX102637 diluted at 1:1000

GTX102637 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
C:MOLT4(GTX27912)
10% SDS PAGE
GTX102637 diluted at 1:500

GTX102637 IHC-P Image

ACAT1 antibody [N1N3] detects ACAT1 protein at cytoplasm on human normal kidney by immunohistochemical analysis.
Sample: Paraffin-embedded human normal kidney.
ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102637 WB Image

Sample (30 μg of whole cell lysate)
A: whole zebrafish
10% SDS PAGE
GTX102637 diluted at 1:1000

REFERENCE

Application Reference

Hiromu Yano et al. Cancer Sci 2022; 113 (6) : 2129-2143 Blocking cholesterol efflux mechanism is a potential target for antilymphoma therapy.

Application : WB

Chimalapati S et al. Elife 2020; 9 Vibrio deploys type 2 secreted lipase to esterify cholesterol with host fatty acids and mediate cell egress.

Application : ICC/IF

Reactivity : Human

Chen JF et al. J Pharm Biomed Anal 2020; 187 : 113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.

Application : WB

Reactivity : Human

Marcela de Souza Santos et al. bioRxiv 2019; (Epub) Vibrio deploys Type 2 secreted lipase to esterify cholesterol with host fatty acids and mediate cell egress.

Application : ICC/IF

Reactivity : Human

Yi-Wen Lo et al. Journal of Cellular and Molecular Medicine 2015; 19 (4) : 744-59 mitochondrial proteomics with sirna knockdown to reveal acat1 and mdh2 in the development of *-resistant uterine cancer

Application : WB, ICC/IF

Reactivity : Human

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