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Cyanine antibody (AP)

Anti-Cyanine antibody (AP) used in Western Blot (WB). GTX27242
Anti-Cyanine antibody (AP) used in Dot blot (Dot). GTX27242

Cat. No. GTX27242

Host

 Sheep

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, Dot, ELISA, IHC

Reactivity

 Species independent
Package
100 μg ($399)
See all Cyanine products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2500
Dot Assay dependent
ELISA 1:2000-1:10000
IHC 1:200-1:1000
Not tested in other applications.

Product Note

Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity against other cyanine analogs is unknown.

PROPERTIES

Form

Liquid

Buffer

50mM Tris-HCl, 150mM NaCl, 1mM MgCl₂, 0.1mM ZnCl₂, 1% BSA, 50% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

A mixture of Cy3 and Cy5 conjugated KLH

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Alkaline Phosphatase (AP)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Cyanine antibody (AP) used in Western Blot (WB). GTX27242

GTX27242 WB Image

Dot Blot of Sheep anti-Cyanine Antibody Alkaline Phosphatase Conjugated. Antigen: Row 1 - Cy3 Streptavidin Conjugated Row 2 - Cy5 Streptavidin Conjugated. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Sheep anti-Cyanine Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT.

Anti-Cyanine antibody (AP) used in Dot blot (Dot). GTX27242

GTX27242 Dot Image

Dot blot analysis of Cy3-conjugated (row 1) and Cy5-conjugated (row 2) streptavidin using GTX27242 Cyanine antibody (AP).
Lane 1 : 200 ng
Lane 2 : 66.67 ng
Lane 3 : 22.22 ng
Lane 4 : 7.41 ng
Lane 5 : 2.47 ng
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399