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Protein G antibody (Biotin)

Anti-Protein G antibody (Biotin) used in Dot blot (Dot). GTX27251

Cat. No. GTX27251

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 Dot, ELISA

Reactivity

 Staphylococcus aureus
Package
1 mg ($399)
See all Protein G products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
Dot Assay dependent
ELISA 1:90000-1:350000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Staphylococcus aureus

Immunogen

Protein G collected from Streptococcus sp

Purification

IgG fraction
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Conjugation

Biotin

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Protein G antibody (Biotin) used in Dot blot (Dot). GTX27251

GTX27251 Dot Image

Dot blot analysis of protein G with (row B) or without (row A) TCEP reduction using GTX27251 Protein G antibody (Biotin).
Lane 1: 100 ng
Lane 2 : 33.33 ng
Lane 3 : 11.11 ng
Lane 4 : 3.7 ng
Lane 5 : 1.23 ng
Dilution : 1 μg/mL

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399